acetaminophen [TUSOM Pharmwiki] Frontiers Acetaminophen induced liver injury: Molecular mechanism and treatments from natural products Tylenol's Impact on Glutathione and Liver Damage Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA Transcription Factor Nrf2 Is Essential for Induction of NAD(P)H:Quinone Oxidoreductase 1, Glutathione S Transferases, and Glutamate Cysteine Ligase by Broccoli Seeds and Isothiocyanates The Journal of Nutrition Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.